Prior to seizing the throne, Ay undertook a crucial duty in the courts of multiple pharaohs, including Tutankhamen and Akhenaten. The male lineage of Ay's royal family soon vanished, however he is considered to be related to the royal family, specifically one of the brothers of Queen Tiye, who was the wife of the famous pharaoh, Amenhotep III. According to engravings from the Amarna period (late 18th dynasty) Tey is referred to as the "nurse of the Great Royal Wife," in which the royal wife is Nefertiti, Ay's rumoured daughter. This suggests that Tey was not Nefertiti's mother, and perhaps she was the second wife of Ay, after the death of Nefertiti's biological mother. However, Ay and Tey are never referred to as the father and mother of Nefertiti. This indicates that Tey's only relation to Nefertiti is by being her wet nurse.
Tey had a daughter with Ay, named Mutbenret, who went on to marry Horemheb, who later became the successor of the throne after Ay. It is also believed that Tey possibly had a sister named Mutemnub.
A dignitary named Ay is called second priest of Amun, high priest of Mut and Steward of Queen Tey on a statue now in the Brooklyn Museum. This man's parents are recorded on the statue as Mutemnub and Nakhtmin. Mutemnub is said to be a sister of Queen Tey, and the inscription is usually interpreted to mean that she was the sister of Tey, wife of Ay.
Tey may have been buried with her husband in WV23, and fragments of female human bones found in the tomb may be Tey's.
There is a statue in the Brooklyn Museum depicting Ay, which refers to him as the "Steward of Queen Tey." This statue is said to have been from 1336-1327 B.C.
Tey is also depicted in a rock chapel dedicated to fertility god Min in Akhmim.